Yes, you will get a noticeably adverse effect, and you could even damage the speakers. The problem is that a speaker will distort badly when it is fed a signal to which it can not respond. Bass probably has the worst effect. If you fed 80 Hz into a speaker that is only rated down to 1 KHz, then you will get massive distortion in the bass that will also distort its midrange output. Trebble might not be as bad, but the higher frequencies fed into that same speaker would produce some distortion, and it could also produce excessive heat in the voice coils which could lead to damage.

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